Transformation of yeast by agitation with glass beads.
نویسندگان
چکیده
We have found that agitation of Saccharomyces cerevisiae with glass beads and plasmid DNA using a vortex mixer results in genetic transformation of the yeast cells. This method is less efficient, but considerably more convenient, than other yeast transformation procedures. The fact that the minimal requirements for transformation are simply physical damage and the presence of DNA in an osmotically supportive environment suggests that this process may occur in nature.
منابع مشابه
A Comparison of Cell Disruption Procedures for the Recovery of Intracellular Carotenoids from Sporobolomyces Ruberrimus H110
Carotenoids are intracellular pigments produced by microorganisms, including some species of yeasts, in the stationary phase of growth by the secondary metabolic pathways. In the present study, different methods of Sporobolomyces ruberrimus H110 yeast cell lysis were evaluated with the objective of optimizing the recovery of intracellular pigments. Three extraction methods were used: vortex (gl...
متن کاملGlass bead transformation method for gram-positive bacteria
A simple, inexpensive and reproducible transformation method was developed for Gram-positive bacteria. It was based on agitation of bacterial protoplasts with glass beads in the presence of DNA and polyethylene glycol. By using this method, introduction of pGK12 into protoplasts of several strains of Gram-positive bacteria was achieved.
متن کاملCopacabana method for spreading E. coli and yeast colonies.
Molecular biology techniques require the isolation of individual clones for analysis after bacterial or yeast transformation. For E. coli and Saccharomyces cerevisiae, the dispersion of colonies on plates has traditionally involved the use of a glass spreader—a bent glass rod that could be repeatedly flame sterilized and used to spread a small aliquot of microorganisms from the center of the pl...
متن کاملEffects of Different Sizes of Glass Beads on the Release of Sporocysts from Eimeria tenella Oocysts
The oocyst wall is severed by means of mechanical injury or chemical agents. This study reports the percentage of in vitro sporocyst release following mechanical shaking in the presence of varying sizes of glass beads. Glass beads measured 0.5, 1, and 3 mm in diameter and were shaken with the oocysts for different times ranging from 5 sec to 5 min. Approximately 80% of sporocysts were released ...
متن کاملSelective and stable adhesion of Yeast cells to Boronate-containing Polymer Grafted to glass Supports
A series of sorbents containing various amounts of borate groups were prepared by chemical immobilization ofP-aminoethylphenylboric acid on a copolymer- treated organosilane spacer glass plates. It was found thatbaker's yeast cells selectively adhered to these supports. Yeast cells stained with procion red HE-3B formeddenser and more stable layer on the copolymer-grafted supports compared onto ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Genetics
دوره 120 3 شماره
صفحات -
تاریخ انتشار 1988